Search results for "Neuron differentiation"

showing 3 items of 3 documents

Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation

2012

Gene expression profiles can be used to infer previously unknown transcriptional regulatory interaction among thousands of genes, via systems biology 'reverse engineering' approaches. We 'reverse engineered' an embryonic stem (ES)-specific transcriptional network from 171 gene expression profiles, measured in ES cells, to identify master regulators of gene expression ('hubs'). We discovered that E130012A19Rik (E13), highly expressed in mouse ES cells as compared with differentiated cells, was a central 'hub' of the network. We demonstrated that E13 is a protein-coding gene implicated in regulating the commitment towards the different neuronal subtypes and glia cells. The overexpression and …

Chromosomal Proteins Non-HistoneCellular differentiationNeurogenesisNerve Tissue ProteinsBiologyCell LineMiceGene expressionProtein Interaction MappingGeneticsTranscriptional regulationmedicineAnimalsGene Regulatory NetworksTransgenesEmbryonic Stem CellsGene Expression ProfilingSystems BiologyNeurogenesisBrainComputational BiologyEmbryonic stem cellCell biologyGene expression profilingmedicine.anatomical_structurenervous systemNeuron differentiationNeurogliaTranscriptome
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PBX1 acts as terminal selector for olfactory bulb dopaminergic neurons

2020

15 páginas, 8 figuras. Supplementary information available online at http://dev.biologists.org/lookup/doi/10.1242/dev.186841.supplemental

MaleInterneuronCell SurvivalNeurogenesisRNA SplicingNeuron differentiationMitosisBiologyAdult neurogenesis03 medical and health sciencesOlfactory bulb0302 clinical medicineNeuroblastInterneuronsmedicineAnimalsProtein IsoformsCell LineageProgenitor cellTerminal selector10. No inequalityMolecular BiologyTranscription factorBody Patterning030304 developmental biologyMice KnockoutDopaminergic neuron0303 health sciencesDopaminergic NeuronsPre-B-Cell Leukemia Transcription Factor 1fungiNeurogenesisDopaminergicCell DifferentiationExonsEmbryo Mammalian3. Good healthOlfactory bulbmedicine.anatomical_structureMutationNeuron differentiationNeuroscience030217 neurology & neurosurgeryTranscription FactorsAlternative splicingDevelopmental BiologyDevelopment
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The expression level of the orphan nuclear receptor GCNF (germ cell nuclear factor) is critical for neuronal differentiation.

2004

The germ cell nuclear factor (GCNF) is essential for normal embryonic development and gametogenesis. To test the prediction that GCNF is additionally required for neuronal differentiation, we used the mouse embryonal carcinoma cell line PCC7-Mz1, which represents an advantageous model to study neuronal cells from the stage of fate choice until the acquirement of functional competence. We generated stable transfectants that express gcnf sense or antisense RNA under the control of a tetracycline-regulated promoter. After retinoic acid-induced withdrawal from the cell cycle, sense clones developed a neuron network with changed properties, and the time course of neuron maturation was shortened.…

Patch-Clamp TechniquesGerm cell nuclear factorSynaptophysinDown-RegulationGene ExpressionReceptors Cytoplasmic and NuclearNerve Tissue ProteinsTretinoinBiologyNestinMiceEndocrinologyGAP-43 ProteinIntermediate Filament ProteinsNuclear Receptor Subfamily 6 Group A Member 1AnimalsRNA AntisenseMolecular BiologyNeuronsCell CycleCell PolarityCell DifferentiationGeneral MedicineCell cycleNestinCell biologyUp-RegulationNeuroepithelial cellDNA-Binding Proteinsnervous systemNeuron maturationSynaptophysinbiology.proteinNeuron differentiationStem cellMicrotubule-Associated ProteinsMolecular endocrinology (Baltimore, Md.)
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